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Hormone‐dependent enhancement of neoplastic phenotype expression by MuMTV‐infected heterologous cells, and MuMTV‐induced cellular antigens
Author(s) -
Kryukova I. N.,
Litvinov S. V.
Publication year - 1984
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910330418
Subject(s) - antigen , heterologous , monoclonal antibody , biology , microbiology and biotechnology , cell , antibody , immunology , biochemistry , gene
A new property, i.e. acquisition of insulin (insulin + dexa‐methasone)‐mediated enhancement of anchorage independence by heterologous MuMTV‐infected cells, is described. Hormone‐mediated anchorage‐independent growth enhancement in MuMTV‐infected cells is followed by expression of a cell‐surface antigen, recognized by allogeneic (C3H→AKR) anti‐Thy 1.2 antigen serum. The absorption test and the use of monoclonal antibody to the Thy 1.2 antigen showed that hormone‐dependent antigen on the cell surfaces of experimentally and naturally MTV‐infected cells was different from the Thy 1.2 antigen itself. Utilization of anti‐p53 ( protein of transformation ) monoclonal antibody enabled us to identify this cell membrane antigen as p53.