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Binding of five monoclonal anti‐CEA antibodies with different epitope specifities to various carcinoma tissues
Author(s) -
Wagener Christoph,
Petzold Petra,
Köhler Wolfgang,
Totović Vladimir
Publication year - 1984
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910330409
Subject(s) - epitope , monoclonal antibody , carcinoembryonic antigen , antibody , antigen , oncofetal antigen , biology , pathology , carcinoma , epithelioma , monoclonal , microbiology and biotechnology , cancer , medicine , immunology , genetics , tumor associated antigen
Five monoclonal antibodies that recognize different epitopes on carcinoembryonic antigen (CEA) were reacted with tissue sections of various carcinoma specimens. The vital, non‐necrotic tissues of those carcinomas of the oesophagus, pancreas, colon and rectum, medullary thyroid, ovary and cervix in which CEA related epitopes were detectable bound all five antibodies to a similar degree. In 3/5 lung carcinomas, 2/10 mammary carcinomas and 1/5 gastric carcinomas, a significantly different binding of the monoclonal antibodies by vital tumour tissue was present as determined by three independent investigators. In necrotic tissue areas, heterogeneity of antibody binding was more common. In a previous investigation, it was shown that normal granulocytes and liver tissue differentially bind the monoclonal anti‐CEA antibodies, indicating the presence of crossreacting antigens. The equivalent binding of the five monoclonal anti‐CEA antibodies to most of the carcinomas tested suggests that this binding is due to the presence of complete CEA molecules and not only of crossreacting antigens.