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Further characterization of marek's disease virus‐infected lymphocytes. II. In vitro infection
Author(s) -
Calnek B. W.,
Schat K. A.,
Ross L. J. N.,
Chen CL. H.
Publication year - 1984
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910330319
Subject(s) - marek's disease , biology , virus , virology , spleen , antigen , in vitro , cell culture , serial passage , incubation , microbiology and biotechnology , immunology , biochemistry , genetics
Lymphocyte cultures from chicken spleens had been shown to be susceptible to in vitro infection by Marek's disease virus (MDV) 4 in an earlier report from this laboratory. In that study, virus infection was evidenced by virus isolation and detection of viral internal antigen (VIA) 2 days post inoculation (DPI), and serial passage was accomplished by adding fresh spleen cells at 2‐day intervals. The susceptible cells were identified as bursa‐derived lymphocytes (B cells). Using a dual fluorescence technique to identify surface markers for B cells, thymus‐derived lymphocytes (T cells) or la antigen on VIA‐positive cells, we have now shown that a small proportion (generally less than 10%) of VIA‐positive cells observed 2 DPI are T cells, and that a low level of infection can be maintained by serial passage of MDV in cultures totally free of B cells. Most infected T cells in this study had Ia antigen. As the incubation period for infected cultures was extended from 2 to 4 or 5 days, the average number of viable cells decreased but the percentage of viable cells infected with MDV (VIA‐positive) increased. Also, both the proportion and the actual number of infected T cells increased, significantly more so in cultures from genetically susceptible P‐2 donors than from resistant N‐2 donors. Spleen‐cell cultures from resistant Line 6 chickens were markedly less susceptible than those from susceptible Line 7 chickens to in vitro MDV infection, as assessed by numbers of VIA‐positive cells at 5 DPI.