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Lysis of fld‐3 friend erythroleukemia cells in vitro and in vivo : Effect of 89 sr treatment and friend virus infection
Author(s) -
Lust John A.,
Bennett Michael,
Kumar Vinay
Publication year - 1984
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910330117
Subject(s) - biology , in vivo , cytotoxic t cell , in vitro , interleukin 12 , microbiology and biotechnology , friend virus , antigen , leukemia , lymphokine activated killer cell , effector , immunology , biochemistry
The effector cells from non‐immunized mice capable of lysing 51 Cr‐labelled FLD‐3 BALB/c Friend virus‐induced erythroleukemia cells in vitro and cells capable of clearing FLD‐3 cells labelled with 5‐iodo‐2′‐deoxyuridine‐ 125 I ( 125 IdUrd) from the lungs in vivo were characterized and compared with natural killer (NK) cells reactive against YAC‐1 lymphoma cells. Unlike NK cells, the cells capable of lysing FLD‐3 cells in vitro were insensitive to antibodies directed agains NK‐2.1 or Thy‐1.2 antigens (plus complement) and to pretreatment of mice in vivo with silica particles, 89 Sr or estradiol. Heat‐killed C. parvum organism stimulated anti‐FLD‐3 effector cells without changing the slow rate (24 h) of lysis in vitro . The ability to clear FLD‐3 and YAC‐1 cells from the lung was normal and defective, respectively, in C57BL/6‐ bg/bg (beige) mice and in mice pretreated with 89 Sr or estradiol. We conclude that natural cytotoxic (NC) cells lyse FLD‐3 cells. Fv‐2 , which regulates resistance to leukemia induction by Friend virus, does not regulate NC(FLD‐3) activity, and the virus does not affect NC(FLD‐3) activity during the first several days of infection of normal genetically susceptible mice. However, infection of 89 Sr‐treated mice inhibits NC(FLD‐3) function owing to the activation of suppressor cells. These data suggest (but do not prove) that effector cells similar or identical to NC(FLD‐3) cells may function in vivo to resist the proliferation/survival of certain leukemia cells.