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Presence of EBV‐DNA sequences in nasopharyngeal cells of individuals without IgA‐VCA antibodies
Author(s) -
Desgranges C.,
Pi G. H.,
Bornkamm G. W.,
Legrand C.,
Zeng Y.,
DeThé G.
Publication year - 1983
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910320504
Subject(s) - serology , antibody , dna , biology , titer , virus , epstein–barr virus , virology , dna sequencing , southern blot , immunology , microbiology and biotechnology , genetics
Exfoliated nasopharyngeal (NP) cells from 62 normal Cantonese Chinese having IgA/VCA antibodies for more than a year and from 39 similar persons without IgA/VCA antibodies, were tested for the presence of EBV/DNA sequences by spot followed by blot hybridization tests, using the cloned internal repeat of B95‐8 viral DNA as probe. Thirteen out of 62 specimens from IgA/VCA‐positive (21%) and six out of 39 specimens (15.4%) from IgA/VCA‐negative individuals were found to contain EBV/DNA sequences. Forty‐six cases (20 IgA/VCA‐positive and 26 IgA/VCA‐negative) were followed a year later for EBV/DNA sequences and EBV serology. Half of the individuals having EBV/DNA sequences in their exfoliated NP cells in 1981 did not have detectable EBV sequences a year later, and to out of 15 negative individuals became EBV/DNA‐positive. There was no obvious correlation between EBV/DNA detectability and EBV serology. (We conclude that the best marker for NPC risk remains the increasing IgA/VCA and/or EA antibody titers.)