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Stable heat‐resistant clones selected from wild‐type and surface variants of B‐16 melanoma
Author(s) -
Tao TienWen,
Calderwood Stuart,
Hahn George M.
Publication year - 1983
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910320502
Subject(s) - cell culture , heat resistance , biology , melanoma , concanavalin a , microbiology and biotechnology , genetics , in vitro , materials science , composite material
Stable heat‐resistant clones were selected from wild‐type B‐16 melanoma cells and from three of their surface variants resistant to the lectins wheat‐germ agglutinin, ricin and concanavalin A. The selection procedure included three or four cycles of heating the cells in culture at 43°C for 2‐1/2 to 3‐1/2 h interspersed with growth at 37°C. The survivability of the heat‐resistant (HR) variant cells at elevated temperatures of 43°C for 160 min and 45°C for 40 min was 2–4 logs greater than that of their respective parents. This acquired property of heat resistance appeared to be a stable phenomenon, persisting in these cell lines for more than 80 generations. One HR variant line carried in tissue culture for 250 generations showed no change in the heat‐resistance characteristic. Acquisition of resistance appeared to be a gradual process with intermediate stages preceding the more pronounced degree of resistance. These newly selected HR variants join the existing surface variants of B‐16 melanoma to result in a large family of variants from the same cell lineage to make this system a powerful tool for studying the relationship between heat sensitivity, metastasis and hyperthermia treatment of cancer.