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Malignant myeloblastic transformation of murine long‐term bone marrow cultures by F‐MuLV: In vitro reproduction of a long‐term leukemogenesis, and investigation of preleukemic events
Author(s) -
Heard Jean Michel,
Fichelson Serge,
Sola Brigitte,
Martial Marie Antoinette,
Berger Roland,
Varet Bruno,
Levy Jean Paul
Publication year - 1983
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910320216
Subject(s) - in vitro , bone marrow , biology , in vivo , immunology , haematopoiesis , malignant transformation , virus , transformation (genetics) , cancer research , virology , microbiology and biotechnology , genetics , stem cell , gene
Abstract A Friend helper virus I −5 (F‐MuLV) which induces my‐eloblastic leukemias in mice after a latency of several months, was used to infect long‐term bone marrow cultures. From 48 to 71 weeks after in vitro infection, 4/14 cultures gave rise to transplantable malignant myeloblas‐tic cells. These cells were shown to genuinely result from an in vitro transformation of virus‐infected normal bone marrow cells. The in vitro transformation reproduced the course of the in vivo disease. It provided unique material for in vitro investigation of the preleukemic stages of long‐term leukemogenesis. Successive cellular events were: (I) freezing of the normal myelomonocytic differentiation process; (2) change from factor‐dependent to an autonomous growth; (3) acquisition of in vivo tumorigenicity.