Variance in resistance to natural and antibody‐dependent cellular cytotoxicity and to complement‐mediated lysis among K562 lines | Zendy

Kimber Ian | Zendy; Moore Michael | Zendy; Roberts Kevan | Zendy

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Variance in resistance to natural and antibody‐dependent cellular cytotoxicity and to complement‐mediated lysis among K562 lines

Author(s) -

Kimber Ian,

Moore Michael,

Roberts Kevan

Publication year - 1983

Publication title -

international journal of cancer

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.475

H-Index - 234

eISSN - 1097-0215

pISSN - 0020-7136

DOI - 10.1002/ijc.2910320213

Subject(s) - cytotoxicity , lysis , antibody dependent cell mediated cytotoxicity , biology , cell culture , k562 cells , effector , natural killer cell , antibody , immunology , microbiology and biotechnology , in vitro , genetics

Isolation of sub‐populations of the NK‐sensitive ery‐thro‐leukaemic cell line K562 by limiting dilution techniques has revealed‐marked clonal variation in susceptibility to natural cytotoxicity. Detailed examination of two such lines (E10/P2 and F9/P2) which differed significantly in their susceptibility to both native and activated NK‐cell‐mediated lysis revealed that their differences were stable and independent of culture conditions. The resistant (F9/P2) and sensitive (E10/P2) lines had comparable cold‐inhibitory and effector‐cell adsorption capacities, indicating that differential susceptibility was not attributable to variable expression of NK target structures. F9/P2 was also less susceptible to antibody‐dependent cellular cytotoxicity (ADCC) and complement‐mediated lysis, indicating the existence of variants with a generalized capacity to resist several immunolytic processes.

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