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Expression of human placental‐type alkaline phosphatase in primary breast cancer
Author(s) -
McDicken I. W.,
Stamp G. H.,
McLaughlin P. J.,
Johnson P. M.
Publication year - 1983
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910320211
Subject(s) - alkaline phosphatase , immunoassay , peroxidase , staining , monoclonal antibody , enzyme , immunohistochemistry , carcinoma , isozyme , chemistry , antibody , microbiology and biotechnology , pathology , biology , medicine , biochemistry , immunology
A monoclonal antibody (H317) with specificity for the heat‐stable placental‐type alkaline phosphatase (Pl‐ALP) isoenzyme has been used to investigate the occurrence of Pl‐ALP in patients with primary breast carcinoma. All pre‐operative plasma samples were negtive for Pl‐ALP in a sensitive solid‐phase enzyme immunoassay with a lower limit of detection of 0.1 U/I. In contrast, using a peroxidase‐anti‐peroxidase staining technique on fixed tissue sections, as well as enzyme immunoassay on fresh tissue extracts, Pl‐ALP could be demonstrated in the carcinomatous tissue of all seven patients investigated.