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Differential cytotoxicity of tumour promoter TPA for EBV‐negative human lymphoma cell lines and their EBV‐converted sublines
Author(s) -
Béchet JeanMarie,
Guétard Denise
Publication year - 1983
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910320110
Subject(s) - lymphoma , cell culture , epstein–barr virus , cytotoxicity , virus , cancer research , antigen , phorbol , biology , tetradecanoylphorbol acetate , cell , in vitro , microbiology and biotechnology , virology , chemistry , immunology , biochemistry , signal transduction , protein kinase c , genetics
Abstract The tumour‐promoting phorbol diester 12‐ O ‐tetradecanoyl‐phorbol‐13‐acetate (TPA) is toxic at nanomolar concentrations for Epstein‐Barr virus (EBV)‐negative human lymphoma cell lines BJAB and Ramos. Sublines that have been converted to Epstein‐Barr nuclear antigen (EBNA)‐positivity by infection with EBV survive and grow in TPA concentrations that are lethal for their non‐converted parental lines. Resistance to the growth‐inhibiting effect of TPA is higher in sublines of BJAB and Ramos that have been converted by the transforming B95‐8 strain of EBV than in those converted by the non‐transforming P3‐HRI strain. The loss of TPA‐sensitivity may reflect a change in the transformation or differentiation state of lymphoma cells as a result of EBV‐conversion.