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Neoplastic transformation of fetal lamb kidney cells by bovine leukemia virus
Author(s) -
Rhim Johng S.,
Kraus Matthias,
Arnstein Paul
Publication year - 1983
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910310620
Subject(s) - biology , bovine leukemia virus , neoplastic transformation , virus , virology , oncovirus , transformation (genetics) , antigen , reverse transcriptase , cell culture , malignant transformation , viral transformation , leukemia , microbiology and biotechnology , cancer , rna , immunology , cancer research , carcinogenesis , genetics , gene
Neoplastic transformation of fetal lamb kidney (FLK) cells by bovine leukemia virus (BLV) is reported. Morphological transformation was observed in FLK cells within a few weeks of BLV inoculation. BLV‐transformed FLK cells had the following properties generally associated with viral transformation: (1) altered morphology; (2) increased growth rate; (3) colony formation in soft agar medium; (4) formation of large cell aggregates and growth in this aggregate form above an agar base; and (5) tumorigenicity in nude mice. The transformed cells contained BLV‐specific p24 and gp51 antigens and were virus producers. BLV was detected in transformed cells by syncytial assay. The transformed cells yielded high‐titered viral reverse transcriptase activity and viral particles. The transformed cells contained marker chromosomes (M 1 , M<2>, M<3>, M 4 and M 7 ) and a large submetacentric chromosome, but control cells did not. Thus, we demonstrated the neoplastic transformation of FLK cells by BLV.