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Biological characterization and clinical applications of a monoclonal antibody recognizing an antigen restricted to neuroectodermal tissues
Author(s) -
Allan Patricia M.,
Garson J. A.,
Harper Edna I.,
Asser Ushi,
Coakham H. B.,
Brownell Betty,
Kemshead J. T.
Publication year - 1983
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910310510
Subject(s) - neuroblastoma , pathology , monoclonal antibody , antigen , bone marrow , biology , antibody , spleen , neuroectodermal tumor , immunohistochemistry , immunofluorescence , immunology , medicine , cell culture , genetics
The monoclonal antibody UJ13A was raised following immunization of mice with human foetal brain and subsequent somatic cell hyridization of spleen cells with the mouse myeloma cell line P3‐X63‐AG8‐653. The antibody is of the IgG 1 subclass and has been shown by indirect immunofluorescence studies on normal foetal, paediatric and adult tissues to selectively bind to most tissues of neuroectodermal origin. Many tumours of neural origin also express the UJI3A antigen and the reagent can be used to distinguish primary intracranial neural tumours from secondary carcinomas and lymphomas. UJI3A is also useful as one of a panel of reagents employed for the identification of metastatic spread of neuroblastoma cells to bone marrow and cerebrospinal fluid. Knowledge of the full spectrum of normal and malignant tissues binding UJ13A suggests that the antibody may have a role in the radioimmunolocalization of neuronal tumours such as neuroblastoma.