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Metastatic clones selected from an RSV‐induced mouse sarcoma share a common marker chromosome
Author(s) -
Di Renzo Maria Flavia,
Doneda Luisa,
Larizza Lidia,
Comoglio Paolo M.
Publication year - 1983
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910310410
Subject(s) - clone (java method) , biology , chromosome , marker chromosome , chromosome instability , fibrosarcoma , phenotype , sarcoma , genetic marker , cell culture , microbiology and biotechnology , cancer research , karyotype , pathology , genetics , dna , medicine , gene
Previous work has shown that the metastatic potential of RSV‐transformed fibroblasts is correlated with the ability to form colonies in 0.6% (“hard”) agar. Metastatic subclones were selected by this property from the nonmetastasizing fibrosarcoma B77‐313 line. A marker chromosome was found at high frequency (90% of cells) in all the subclones studied. This marker was detectable in only 0.5% of the parental B77‐3T3 cells, demonstrating that metastatic clone precursors pre‐existed, as a small minority, in the parental line. The genotypic marker appeared to be steadily associated with the metastatic phenotype since, after prolonged in vitro propagation, the subclones retained both the marker chromosome and the high metastatic potential. Although the marker chromosome was constantly present, chromosomal numerical and structural aberrations were also found in 20% of the long‐term‐propagated subclone cells, supporting the suggestion that metastatic properties are associated with cytogenetic instability.