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Lectin‐resistant variants of mouse melanoma cells. II. In vitro characteristics
Author(s) -
Tao TienWen,
Jenkins Jennifer M.,
Vosbeck Klaus,
Matter Alex,
Miller Marcia,
Jockusch Brigitte M.,
Shen ZhongHou,
Burger Max M.
Publication year - 1983
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910310218
Subject(s) - lectin , in vitro , wheat germ agglutinin , concanavalin a , melanoma , in vivo , biology , peanut agglutinin , microbiology and biotechnology , melanin , metastasis , cancer research , biochemistry , genetics , cancer
Lectin‐resistant variants of B‐16 melanoma cells were selected with wheat‐germ agglutinin, ricin and concanavalin A. They exhibited altered metastasizing capacity and tumorigenicity in C57BL mice. Several in vitro properties were defined and compared including homotypic adhesiveness, microfilament organization, melanin release, activity of tyrosine hydroxylase, DNA content, and karyotypes. The possible relevance of these properties in vitro for the malignant behavior displayed in vivo is discussed. The usefulness of this approach of selecting surface variants to study the problem of metastasis is also discussed.