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Establishment and characterization of a human non‐secretory plasmacytoid cell line and its hybridization with human B cells
Author(s) -
Ritts R. E.,
RuizArgüelles Alejandro,
Weyl Karin G.,
Bradley Anne L.,
Weihmeir Bernadette,
Jacobsen Deborah J.,
Strehlo Bonnie L.
Publication year - 1983
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910310202
Subject(s) - biology , clone (java method) , polyploid , cell culture , in situ hybridization , phenotype , mutant , microbiology and biotechnology , genetics , gene , ploidy , gene expression
A human non‐secretory plasmacytoid cell line has been established for 6 years in more than 170 passages. Over 300 passages have been made from several early and late passages. The cell line is karyotypically normal, easily grown and has the characteristic features of a non‐secretory plasmablast. Its characteristics suggest its use for hybridization by new methods as well as a study of its secretory defect. HPRT‐negative phenotypic mutants can be derived from this line and a single polyploid clone has also been isolated. Hybridization with the HPRT+ and HPRT‐ lines X human B cells is described.