Premium
Methods and mechanisms for the development of MTX resistance in human lymphoid cells
Author(s) -
Alam A.,
Geddes A. J.,
North A. C. T.
Publication year - 1983
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910310108
Subject(s) - methotrexate , dihydrofolate reductase , antifolate , cell culture , biology , cancer research , antimetabolite , immunology , chemistry , genetics
Conditions are described for the development of methotrexate resistance in CCRF/CEM human lymphoid cells and an attempt is made to distinguish between those conditions that lead to methotrexate resistance through impairment of cellular transport of methotrexate and those that lead to resistance through enhanced production of dihydrofolate reductase. A restistant line showing a 200‐fold enhancement of dihydrofolate reductase has been obtained. The CCRF/CEM human cells are much more sensitive to methotrexate than are murine cells.