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Effect of elicitation on peritoneal macrophage subpopulations: Size distributions, ectoenzyme phenotypes and antitumor activity
Author(s) -
Morahan Page S.,
Rozner Marc A.,
Jessee Ellen J.
Publication year - 1982
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910300618
Subject(s) - ficoll , macrophage , population , biology , phenotype , in vitro , cell , differential centrifugation , centrifugation , immunology , microbiology and biotechnology , chemistry , biochemistry , medicine , peripheral blood mononuclear cell , environmental health , gene
Abstract Resident (R), Brewer's thioglycollate broth (TG) and C. parvum (CP)‐elicited murine peritoneal cell populations were separated into subpopulations by centrifugation on discontinuous Ficoll gradients consisting of layers of 4, 6, 8 and 10% Ficoll. The resulting subpopulations were shown to be distinct on the basis of cell size, ectoenzyme phenotypes and antitumor activity. The cell size distributions were analyzed by means of a Coulter channelyzer and software developed for a computer. The 4–6% Ficoll interface fraction comprised the smallest macrophages, with most cells in this subpopulation appearing to range in cell volume from 150–275 μm 3 . The largest sized macrophages were found in the 10%‐pellet fraction, with most cells appearing to range in cell volume from 300–600 μm 3 . The ectoenzyme phenotypes of the R, TG and CP unseparated macrophage populations were significantly different. Moreover, the ectoenzyme phenotypes of the smallest, (4–6% Ficoll interface) subpopulation in the R and CP macrophages differed from the other subpopulations. Reduction in alkaline phosphodiesterase I (APD‐I) ectoenzyme activity (as compared with unseparated R macrophages) appeared to be a marker for acquisition of antitumor activity. The small CP macrophages (4–6% Ficoll interface) showed no antitumor activity while the unseparated CP macrophages and all other CP macrophages subpopulations exhibited antitumor activity. The CP macrophage unseparated population and the subpopulations with antitumor activity expressed reduced ADP‐I activity. Conversely, the R or TG macrophage unseparated populations and their subpopulations, along with the CP macrophage 4–6% subpopulation, lacked antitumor activity and failed to show a change in ADP‐I ectoenzyme activity.