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A monoclonal antibody marker of human primitive endoderm
Author(s) -
Williams L. K.,
Sullivan A.,
McIlhinney R. A. J.,
Neville A. M.
Publication year - 1982
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910300609
Subject(s) - monoclonal antibody , immunogen , endoderm , biology , antibody , antigen , teratoma , glycoprotein , microbiology and biotechnology , pathology , immunology , cellular differentiation , biochemistry , medicine , gene
Monoclonal antibodies were raised against the human germ‐cell tumor line, LICR LON HT39/7. The cells of this human embryonal carcinoma cell line were first fixed in formol saline and then dehydrated prior to immunization so that antibodies were produced which reacted with antigens surviving conventional histological processing. Eleven hybrid clones secreted antibody that reacted with both formol saline‐fixed and living cells of the immunogen. One of these antibodies, LICR LON FC 10.2, has been found to react with a membrane glycoprotein of molecular weight 200,000 restricted in distribution to the surfaces of undifferentiated teratoma‐derived cell lines, the luminal surface of human fetal intestine and bronchus and areas of putative endodermal differentiation within germcell neoplasms.