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Immunoglobulins reactive to carcinoembryonic antigen and their relationship to the antigen in malignant ascitic fluid of ovarian carcinoma
Author(s) -
Hill R.,
Khoo S. K.,
Daunter B.,
Silburn P. A.,
Mackay E. V.
Publication year - 1982
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910300509
Subject(s) - carcinoembryonic antigen , antibody , radioimmunoassay , antigen , oncofetal antigen , medicine , ascites , immunology , ovarian carcinoma , carcinoma , pathology , chemistry , ovarian cancer , monoclonal antibody , cancer , tumor associated antigen
Abstract Samples of malignant ascitic fluid from 30 patients with advanced ovarian carcinoma were examined for the presence of IgM antibodies to CEA and PEG‐precipitable proteins binding to 125 I‐CEA. The IgM antibodies to CEA were measured by a solid‐phase radioimmunoassay using ovarian CEA. There was no correlation between the level of IgM antibodies to CEA and that of total IgM in the fluid. In 11 of 30 (37%) samples tested, significant amounts of IgM antibodies to CEA were found. The CEA‐binding proteins were measured by the ability of ascitic fluid to incorporate 125 I‐colonic‐CEA into PEG‐precipitable complexes. In 9 of 39 (30%) samples, the precipitation was significant. There was no association between antibodies to the ABO and Lewis blood group factors and these antibodies to CEA. An inverse relationship was observed between the level of CEA and that of CEA‐binding proteins shown by the two assays. When 125 I‐CEA was incubated with these “positive” samples, a high molecular weight fraction was demonstrated by chromatography. By contrast, in the “negative” samples, there was no incorporation of 125 I‐CEA. These findings would indicate the presence of CEA‐reactive proteins possibly existing as immune‐complex‐like material in ascitic fluid of some patients.