Epstein‐Barr virus strain‐specific differences in transformed cell lines demonstrated in growth characteristics, induction of viral antigens and ADCC susceptibility

Paired lymphoid cell lines were established by transformation with both B95‐8 (B) and QIMR‐WIL (Q) EBV strains, of cells either from cord blood or from peripheral blood or spleens of patients with leukemias or lymphomas. The morphologies of the transformed cell clumps differed consistently between B‐transformed lines (B‐lines) and Q‐transformed lines (Q‐lines) even after 1 year in culture. When the B‐ and Q‐lines were compared for superinfection with P3HR‐1 EBV, B‐lines had a higher frequency of EA induction than did the Q‐lines. The shape of dose‐response curves for superinfection indicated that a much higher multiplicity of infection by P3HR‐1 EBV was required for EA expression in Q‐lines than in B‐lines. This difference in superinfection was independent of the EBV receptor concentration on the two types of lines and reflected apparent control of the level of EA induction by the resident EBV genome of the transformed line. Transforming EBV could be rescued by P3HR‐1 EBV superinfection of both B‐ and Q‐lines originating from cells of patients with Hodgkin's disease and hairy cell leukemia but not from cord blood. The cord blood lines transformed with virus produced spontaneously from these B‐ or Q‐lines, showed that the cell lines contained antigen‐determining information of the resident genome in the original B‐ or Q‐lines, respectively. Superinfected B‐lines were more susceptible to ADCC with anti‐EBV‐positive sera than were superinfected Q‐lines. These experiments demonstrate that distinct biology. differences exist in paired cell lines transformed by different EBV strains.