Premium
Abrogation of the in vitro generation of the cytotoxic T‐cell response to a murine tumor: The role of suppressor cells
Author(s) -
Frost Philip,
Prete Pamela,
Kerbel Robert
Publication year - 1982
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910300214
Subject(s) - cytotoxic t cell , suppressor , in vitro , myeloid derived suppressor cell , cancer research , biology , immunology , microbiology and biotechnology , cancer , biochemistry , genetics
A reproducible in vitro assay for the effect of suppressor T cells on the generation of an in vitro cytotoxic response to a metastatic murine tumor is described. Suppression in this system is maximal. The model uses splenic T cells from DBA/2 mice bearing the MDAY‐D2 metastatic tumor as suppressors of the in vitro generation of a cell mediated cytotoxic response by syngeneic tumor‐bearer ( i.e. , primed) peripheral lymph node cells stimulated with mitomycin C‐treated MDAY‐D2 tumor cells. The effect of the cell mediated cytotoxic response is profound, specific and mediated by splenic T cells. The suppressor T cells appear to impair precursor rather than mature cytotoxic cells. A highly immunogenic variant of MDAY‐D2, namely MDWI, does not generate a suppressor response. Suppressor T cells from an MDAY‐D2 tumor‐bearer cannot suppress the in vitro response to MDWI. This model of potent suppressor T‐cell action is highly reproducible and offers an opportunity for the analysis of why some tumors generate an effective cell mediated cytotoxic response which others stimulate dramatic suppressor T‐cell response.