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Demonstration of the tumorigenicity of transformed rat kidney cell‐lines by intravenous allotransplantation in the neonate
Author(s) -
Hard Gordon C.
Publication year - 1982
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910300212
Subject(s) - mesenchymal stem cell , transplantation , cell culture , pathology , cell , kidney , biology , in vivo , malignancy , cancer research , medicine , endocrinology , genetics , microbiology and biotechnology
The tumorigenic potential of allogeneic rat kidney cell‐lines transformed in vivo by a carcinogenic dose of dimethylnitrosamine (DMN) was investigated by intravenous injection of single cell suspensions into neonatal outbred rats within 24 h of birth. The cell‐lines tested included mesenchymal populations obtained from DMN‐induced renal mesenchymal tumors (RRMT‐2,‐8,‐9) and transformed mesenchymal (TRKM‐5,‐7,‐8) and epithelial (TRKE‐1) cell‐lines derived from the kidneys of rats treated only hours previously with the carcinogen. Additionally, spontaneous nephroblastoma‐derived embryonal cell‐lines (REN‐1,‐2) were included in the study to extend the potential of the neonatal rat system over a range of differentiation lineages. The transplantation system proved to be a rapid and efficacious assay for demonstrating the malignancy of the mesenchymal and embryonal cell‐lines. The major sites for tumor growth were the lungs, heart and eye, but differences in organ predilection were observed for individual cell‐lines. The transformed epithelial cell‐line (TRKE‐1) proved refractory to the single intravenous inoculation but was transplantation‐positive when a follow‐up subcutaneous dose was administered several days later. The resultant growths produced by the diverse differentiation lineages were histologically characteristic for the tumor tissue affiliate of each cell‐line. The results demonstrate the utility of this transplantation system for testing the malignant potential of morphologically transformed cells across the allogeneic barrier as well as proving that DMN is capable of inducing malignant transformation in both mesenchymal and epithelial cell types of the rat kidney after a very short period of exposure in vivo .