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Quantitation of proliferative and cytotoxic precursor cells directed against human tumours: Limiting dilution analysis in peripheral blood and at the tumour site
Author(s) -
Vose Brent M.
Publication year - 1982
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910300202
Subject(s) - cytotoxic t cell , t lymphocyte , cytotoxicity , tumor infiltrating lymphocytes , immunology , lymphocyte , peripheral blood , interleukin 2 , biology , flow cytometry , limiting , microbiology and biotechnology , in vitro , cytokine , immunotherapy , immune system , biochemistry , mechanical engineering , engineering
Blood and tumour‐infiltrating lymphocytes (TIL) from 16 cancer patients have been examined under limiting dilution conditions to determine the frequency of cells responding in mixed tumour‐lymphocyte cultures (MLTC) to autologous tumour and Interleukin‐2 (IL‐2). Tumour‐derived lymphocytes showed a high spontaneous response to IL‐2 alone 1/1,900 in TIL; 1/6,000 in PBL suggesting the presence of “activated” T cells in situ. Proliferative frequencies were increased in MLTC in both blood (1/3,779) and TIL (1/1,084). Phenotypic analyses showed that total T‐cell contents of the responder populations were comparable but TIL were enriched for the OKT 8 + subset with a corresponding reduction in OKT 4 + . TIL showed increased numbers of OKMI + and Tac + lymphocytes. The major cytotoxic precursor expanding under these conditions was reactive against autologous tumour. K562 (NK) were present at a lesser frequency ‐particularly in TIL The data show a concentration and activation of reactive lymphocytes at the tumour site and establish conditions for the clonal expansion of specifically cytotoxck T cells.