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Growth control and cell spreading: Differential response in preneoplastic and in metastatic cell variants
Author(s) -
Raz Avraham,
BenZe'ev Avri
Publication year - 1982
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910290619
Subject(s) - fibrosarcoma , cell , cell growth , melanoma , 3t3 cells , biology , cell culture , pathology , chemistry , cancer research , biophysics , microbiology and biotechnology , medicine , genetics , transfection
Growth control and sensitivity to changes in cell shape were studied in anchorage‐dependent mouse fibroblasts (diploid fibroblasts, 3T3 and 3T6), in DNA tumor‐virustransformed mouse fibroblasts (SVPy 3T3), in four B16 melanoma and five uv‐2237 fibrosarcoma cell variants that exhibit distinct metastatic properties. Differential adhesive conditions were established by precoating the plastic plates with poly (2‐hydroxyethylmethacrylate) that allowed an accurate and reproducible control of cell shape, from flat to spherical. Mouse fibroblasts that form a continuum between rigorously controlled cells to fully anchorage‐independent cells, display a direct correlation between degree of growth control and sensitivity to changes in cell spreading. In contrast, there is no apparent direct correlation between sensitivity of growth control to changes in cellular configuration and the metastatic potential of tumor cells.