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Detection of tumor antigens by syngeneic antiserum on in vitro , transformed tracheal epithelial cell line 2‐10‐1
Author(s) -
Braslawsky G. R.,
Kennel S. J.,
Steele V.,
Nettesheim P.
Publication year - 1982
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910290613
Subject(s) - antigen , in vitro , biology , cell culture , antiserum , immune system , malignant transformation , cell , transplantation , immunology , antibody , microbiology and biotechnology , cancer research , medicine , genetics
Abstract Sera obtained from rats showing transplantation immunity to syngeneic, malignant epithelial cell line, 2‐10‐1, were used to identify antigens associated with transformation in vitro . The 2‐10‐1 cell line was derived from exposure of tracheal explants, in vitro , to the carcinogen MNNG. Tumorigenic passages of the cell line were shown to have common antigenic determinants, shared by independently transformed malignant tracheal epithelial cell lines, as well as antigenic determinants that appear to be limited to the immunizing cell line, 2‐10‐1. Care was taken to ensure that antibodies were not produced against viral antigens or non‐specifically absorbed serum components. Early passages of the 2‐10‐1 cell line were not tumorigenic in athymic BALB/c (nu/nu) mice, but became malignant with serial passage in vitro. Antigenic specificities recognized by 2‐10‐1 immune sera were also present on earlypassage 2‐10‐1 cells that had not as yet acquired the malignant phenotype. Such antigen expression must be an early event in neoplastic development.