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Tumorigenicity of human bk papovavirus plaque isolates, wild‐type and plaque morphology mutant, in hamsters
Author(s) -
Watanabe Sumie,
Kotake Setsuko,
Nozawa Akemi,
Uchida Seijiro,
Muto Takeshi
Publication year - 1982
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910290515
Subject(s) - papovavirus , mutant , hamster , biology , virology , microbiology and biotechnology , wild type , virus quantification , mesocricetus , gene , genetics , virus
Abstract The polyoncogenic prototype human papovavirus BKV (Gardner's strain) produced clear, small and large plaques. Two small‐plaque isolates ( wt ‐500 and wt ‐502), as well as a large‐plaque‐forming isolate ( wt ‐501), induced frequent brain tumors and occasional osteosarcomas (but no insulinomas) in hamsters. The large‐plaque former ( wt ‐501) was more tumorigenic than the two small‐plaque isolates. One small ( pm ‐522) and one large turbid plaque mutant ( pm ‐525), which had been rescued from hamster tumor cell lines and have small deletion near the origin of DNA replication, induced frequent brain tumors and insulinomas. Mutant 522 was about five times more tumorigenic than wt ‐501. These results support the hypothesis that the polyoncogenicity of prototype BKV is accounted for by the presence of BKV mutants.