Premium
Establishment and characterization of a human myeloma cell line (KMM‐1)
Author(s) -
Togawa Atsushi,
Inoue Nobumasa,
Hyodo Huminori,
Namba Masayoshi,
Miyamoto Kanji
Publication year - 1982
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910290502
Subject(s) - clone (java method) , plasmacytoma , cell culture , biology , microbiology and biotechnology , karyotype , endoplasmic reticulum , bence jones protein , antigen , plasma cell , antibody , doubling time , immunoglobulin light chain , multiple myeloma , immunology , biochemistry , chromosome , genetics , dna , gene
Abstract A new cell line (KMM‐1) was established from a subcutaneous plasmacytoma of a 62‐year‐old male with multiple myeloma. Immunological studies indicated that cultured cells were derived from the same clone of myeloma cells in vivo : smeared cells were stained with fluoresceinconjugated globulin of antisera monospecific to lambdachain, and lambda‐chains in the cell extracts and in culture media were identical to the Bence‐Jones protein found in the patient's urine. The cell line grew in suspension with a doubling time of 36‐40 h and contained primitive plasmablastoid cells with prominent nucleoli and rough endoplasmic reticulum. Cells had the karyotype of 47, X, ‐Y. lq+, ‐2, +t(1:2) (cen:cen), +7, 12q+, 14q+, +mar and carried no Epstein‐Barr virus‐determined nuclear antigen. Surface markers were as follows; E rosette (‐), IgG Fc receptor (‐), C3 receptor (‐), S‐lg (+), TdT (‐), asialo‐GM, (‐). The reasons for the successful establishment of the myeloma cell line are discussed.