Premium
Dissection of the antigenic determinants expressed on the cell surface of rsv‐transformed fibroblasts by monoclonal antibodies
Author(s) -
Ferracini Riccardo,
Prat Maria,
Comoglio Paolo M.
Publication year - 1982
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910290419
Subject(s) - rous sarcoma virus , biology , baby hamster kidney cell , virology , antigen , monoclonal antibody , antibody , cell fusion , epitope , hamster , virus , fibroblast , cell culture , microbiology and biotechnology , immunology , genetics
Fusion of P3/X63‐Ag8 mouse myeloma cells with splenocytes obtained from mice hyperimmunized with a BHK hamster fibroblast line transformed by an envstrain of Rous sarcoma virus (RSV) resulted in the production of antibody‐secreting hybridomas. Seven hybrid clones secreted antibodies binding to RSV‐transformed BHK fibroblasts but not to the parental control non‐transformed line. The antibodies produced by three of these clones did identify antigenic determinants expressed also on BHK cells transformed by SV40. The antibodies produced by four other clones reacted specifically with cells transformed by RSV but not with cells productively infected by the transformation‐defective Rous‐associated virus‐1; one of these reacted with RSV‐transformed hamster cells only, three others identified antigenic determinants common to RSV‐ transformed cells of different animal species. These data give further support to the idea that RSV‐transformed cells express a cell‐surface antigen, specific for transformation, the expression of which is controlled by the transforming src gene, and that the specificities carried on it follow a complex pattern, arising from the interaction between antigenic determinants of cellular and viral origin.