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Cross‐reactions between tumor cells and allogeneic normal tissues. inhibition of a syngeneic lymphoma outgrowth in h‐2 and non‐h‐2 alloimmune balb/c mice
Author(s) -
Parmiani Giorgio,
Luisa Sensi M.,
Carbone Giusi,
Colombo Mario,
Pierotti Marco,
Ballinari Dario,
Hilgers Jo,
Hilkens John
Publication year - 1982
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910290316
Subject(s) - lymphoma , cancer research , immunology , neoplasm , transplantation , balb/c , biology , medicine , pathology , immune system
To test whether alloimmunization with H‐2 or/and non‐H‐2 different normal tissues may increase the immunity to syngeneic tumors, groups of BALB/c ( H‐2 d ) mice were immunized with a series of allogeneic lymphoid cells and then challenged i.p. with syngeneic lymphoma cells. The outgrowth of otherwise lethal doses of the Moloney virus‐induced lymphoma YC8 and of its clones was inhibited in BALB/c mice immune to DBA/2 ( H ‐2 d ), C3Hf ( H ‐2 k ), C3H.SW ( H ‐2 b ), C3H.OH ( H ‐2 o 2 ) and to B10 background tissues but not in mice immunized to A/He, BALB.K ( H ‐2 k ) or BALB.B ( H ‐2 b ) normal tissues. Anti‐YC8 effect was also induced by immunizing BALB/c recipients with a pool of five different allogeneic cell lines which included C3Hf, C57BL/6J ( H ‐2 b ), N:NIH ( H ‐2 q ), B10.M ( H ‐2 f ), and DBA/2 lymphoid cells. No growth inhibition of other BALB/c lymphomas induced by Moloney virus (LSTRA), X‐rays (RL♂I) or urethane (UR‐I) was evident in alloimmune mice. In vivo transfer of growth inhibition of YC8 was obtained with BALB/c anti‐B10.D2 peritoneal exudate cells in a Winn assay. The ability of these alloimmune lymphoid cells to delay significantly the survival time of BALB/c mice injected with the mixture of immune cell and YC8 cells was abrogated by anti‐Thy 1.2 plus C' treatment. In addition, nu/nu BALB/c mice were unable to develop resistance to YC8 outgrowth after alloimmunization. The results of this study show that: (1) syngeneic growth of a lymphoma can be prevented by alloimmunization with normal cells; (2) this cross‐reaction involved non‐H‐2 antigens; (3) the phenomenon appeared to be mediated by T cells.