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Syngeneic and allogeneic cell‐mediated cytotoxicity against marek's disease lymphoblastoid tumor cell lines
Author(s) -
Schat K. A.,
Shek W. R.,
Calnek B. W.,
Abplanalp H.
Publication year - 1982
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910290213
Subject(s) - cytotoxicity , marek's disease , biology , virology , cell culture , antigen , histocompatibility , virus , immunology , minor histocompatibility antigen , spleen , lymphoblast , major histocompatibility complex , microbiology and biotechnology , in vitro , human leukocyte antigen , genetics
Cell‐mediated cytotoxicity (CMC) of lymphocytes obtained from chickens infected with Marek's disease (MD) virus against allogeneic MD lymphoblastoid cell lines has been reported by several research groups. Recently, we established a number of cell lines from MD tumors obtained from highly inbred chickens and characterized for major and minor histocompatibility antigens. Allogeneic versus syngeneic CMC was studied using those cell lines and lymphocytes obtained from chickens 6‐8 days post infection with 5B‐1, a non‐oncogenic MD virus. Allogeneic cytotoxicity could be easily demonstrated, while syngeneic cytotoxicity was a rare event. However, increase of the CMC assay period from 4 to 8 h did enhance syngeneic cytotoxicity. Cold inhibition assays demonstrated that the allogeneic cytotoxicity was directed against alloantigens present on spleen lymphocytes sharing the same major histocompatibility antigens as the target cells. Cytotoxicity was not influenced by the sex of either target or effector cells or by the level of virus infectivity of the effector cells.