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Modulation of T leukaemic cell phenotype with phorbol ester
Author(s) -
Delia D.,
Greaves M. F.,
Newman R. A.,
Sutherland D. R.,
Minowada J.,
Kung P.,
Goldstein G.
Publication year - 1982
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910290106
Subject(s) - biology , terminal deoxynucleotidyl transferase , cell culture , cellular differentiation , monoclonal antibody , retinoic acid , cell growth , phenotype , microbiology and biotechnology , biochemistry , antibody , immunology , apoptosis , genetics , gene , tunel assay
A panel of monoclonal antibodies and other markers ( e.g. , terminal deoxynucleotidyl transferase, sheep erythrocyte rosettes, peanut agglutinin) have been used in conjunction with flow cytometry and biochemical analysis to monitor the induction of maturation in human thymic (T) leukaemic cell lines by phorbol ester (TPA). Seven cell lines underwent multiple phenotypic alterations in response to TPA but were unresponsive to synthetic thymic hormones (TP5, FTS) or to other compounds ( e.g. DMSO, retinoic acid) which induce maturation in other types of leukaemia. The changes parallel those observed in normal T‐cell differentiation and partly reflect alterations in glycosyl transferase activity, altered synthesis of proteins and regulation of cell surface receptors (for transferrin) associated with rapid growth and metabolism. These studies further illustrate the reversibility of maturation arrest in human leukaemia and provide support for the view that leukaemia may involve regulatory defects in the coupling of proliferation and maturation. Induction or promotion of terminal differentiation in leukaemic equivalents of T‐cell precursors may provide a convenient system for the study of biochemical and molecular events involved in T‐cell development and diversification.