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Morphological and microbiological signs of endogenous C‐virus in human oocytes
Author(s) -
Larsson E.,
Nilsson B. Ove,
Sundström P.,
Widéhn Sibylle
Publication year - 1981
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910280504
Subject(s) - follicular fluid , perivitelline space , endogeny , oocyte , reverse transcriptase , biology , virus , follicular phase , endogenous retrovirus , andrology , budding , virology , microbiology and biotechnology , embryo , endocrinology , rna , zona pellucida , biochemistry , medicine , genome , gene
Human oocytes in different stages of maturation were obtained by follicular aspiration from women given Clomovid and Gonadex. Particles similar to type‐C virus were observed in three out of 16 oocytes. The particles were irregularly distributed along the oocyte membrane. They were seen both in a state of budding and lying free in the perivitelline space. Reverse transcriptase activity was detected in three out of nine samples of follicular fluid obtained from women other than those donating the oocytes. The supernatants from bat lung cells and dog thymus cells cultivated with oocytes or follicular fluids were tested for reverse transcriptase. An increased activity was observed only transiently in one case. It is assumed that these findings indicate the expression of endogenous retroviruses in human oocytes.