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Epstein‐Barr virus strain‐ and group‐specific antigenic determinants detected by monoclonal antibodies
Author(s) -
MuellerLantzsch Nikolaus,
GeorgFries Brigitte,
Herbst Hermann,
Hausen Harald Zur,
Braun Dietmar G.
Publication year - 1981
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910280311
Subject(s) - monoclonal antibody , antigen , serology , virology , antibody , immunofluorescence , immunoprecipitation , virus , epitope , microbiology and biotechnology , biology , monoclonal , epstein–barr virus , immunology
In order to differentiate Epstein‐Barr virus (EBV) strains by serological markers we prepared hybridomas producing monoclonal antibodies against polypeptides of the QIMR‐WIL EBV strain. These monoclonal antibodies were screened by indirect immunofluorescence techniques. Immunoprecipitation, using EBV‐positive monoclonal antibodies and the protein A method, of 125 I‐labelled polypeptides from purified QIMR‐WIL EBV particles, revealed that a series of monoclonal antibodies against the four main surface and envelope polypeptides precipitating p 340 , p340/p240, p140, and p80 were obtained. When 125 I‐labelled polypeptides from purified P3HR‐I and B95‐8 EBV particles were immunoprecipitated, it could be demonstrated that several anti‐p340 (QIMR‐WIL) antibodies recognized strainspecific antigenic determinants, while anti‐p340/p240 (QIMR‐WIL) as well as anti‐p140 (QIMR‐WIL) antibody clones reacted with antigenic sites which are in common wither among B95‐8 or in addition to P3HR‐I polypeptides. Thus, monoclonal antibodies now provide a serological basis for EBV typing.