Premium
Modulation of the expression of alkaline phosphatase genes in a human malignant cell line passaged through nude mice
Author(s) -
Benham Frances J.,
Balaban Gloria,
Boccelli Denise,
Harris Harry
Publication year - 1981
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910280302
Subject(s) - alkaline phosphatase , cell culture , gene , biology , gene expression , microbiology and biotechnology , cancer research , enzyme , genetics , biochemistry
Hep 2/5 cells, a clone of HeLa which expresses both placental‐type alkaline phosphatase and fetal intestinal alkaline phosphatase (ALP), were grown as solid tumors in nude mice. Analysis of ALP isozymes present in tumor specimens excised at intervals over a 52‐day period showed that levels of placental‐type ALP had dropped markedly, while levels of the intestinal ALP remained fairly constant. Cells re‐cultured from two two separate tumors regained most of the original placental ALP levels. However, levels of the fetal intenstinal ALP in cells returned to culture rapidly increased to more than six times the levels in the original cultured cells, and these increased levels persisted. Passage through nude mice thus induced at least semi‐permanent changes in the level of expression of fetal intestinal ALP in Hep 2/5 cells, and the nature of these changes may relate to the role of the isozyme locus in malignant cells.