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Studies on lung tumours. V. Susceptibility of mice to dimethylnitrosamine‐induced tumour formation in relation to H ‐2 haplotype
Author(s) -
den Engelse L.,
Oomen L. C. J. M.,
Valk M. A. Vander,
Hart A. A. M.,
Dux A.,
Emmelot P.
Publication year - 1981
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910280214
Subject(s) - strain (injury) , haplotype , lung , microbiology and biotechnology , biology , inbred strain , immunology , genetics , gene , medicine , genotype , anatomy
Male mice of the strain A/Sn, of its congeneic partner strain A.SW, C57BL/105cSnA (abbreviated: B10), and of two congeneic strains on a B10 background (B10.A and B10.AKM) were investigated for their susceptibility to lung tumour induction by dimethylnitrosamine (DMN) administered either by intraperitoneal injection or in the drinking water. Strain B10 (haplotype H ‐23) proved to be very resistant, whereas strains A/Sn ( H ‐ 2 b ) and A.SW ( H ‐ 2 s ) were highly susceptible. The introduction of the haplotypes H ‐ 2 a and H ‐ 2 m in the resistant strain B10 resulted in a significant increase in sensitivity towards DMN‐induced lung tumour formation. Lung tumour incidences in male (B10.A × B10)F 1 , hybrids, receiving DMN in drinking water, were found to be intermediate between, and significantly different from, the incidences of identically‐treated parent strains B10.A and B10. Males of back‐cross (B10.A × B10) × B10 BC 1 proved to be of low susceptibility to lung tumour formation by DMN, tumour incidence being very low and not significantly different from that observed in identically‐treated B10 males. It is concluded that, at least in the model system of B10‐derived congeneic strains, H ‐ 2 haplotype is one of the factors important in determining susceptibility towards DMN‐induced lung tumours. Comparison of C3Hf ( H ‐ 2 k ), C3H/Sn ( H ‐ 2 k ) and the latter's congeneic strains C3H.B10 ( H ‐ 2 b ) and C3H.NB ( H ‐ 2 p ) of male mice showed that these strains were moderately susceptible to both lung tumour and hepatoma formation by DMN. Accordingly, the presence of an H ‐ 2 haplotype from a lung‐tumour‐resistant strain ( H ‐ 2 b , B10) on the background of a strain of intermediate susceptibility (C3H) does not decrease susceptibility to lung tumour formation. The results were considered in the light of the H ‐ 2 haplotype dependence of spontaneous lung tumours, and consequently attention has been paid to the histological types of the induced and spontaneous lung tumours.