Premium
Mouse fibroblasts transformed by rous sarcoma virus express a virus‐specific non‐virion transplantation antigen
Author(s) -
Prat Maria,
Tarone Guido,
Comoglio Paolo M.
Publication year - 1981
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910270611
Subject(s) - rous sarcoma virus , biology , virology , antigen , transplantation , virus , hyperimmunization , hamster , microbiology and biotechnology , gene product , immunology , gene , gene expression , medicine , biochemistry , surgery
Rous sarcoma virus (RSV)‐transformed fibroblasts from different animal species a serologically detectable virus‐induced non‐virion cell surface antigen (VCSA), whose expression is controlled by the transforming viral srcgene and by a cellular gene. Now, by in vivo immunization, we have found that RSV‐transformed fibroblasts from different mouse strains share a virus‐specific transplantation antigen. In fact, only animals immunized with irradiated syngeneic or allogeneic fibroblasts transformed by RSV, but not animals immunized with cells transformed by different oncogenic agents, rejected a lethal dose of syngeneic RSV‐induced tumor cells. Immunoprecipitation tests with monospecific antisera showed that the expression of this antigen did not correlate with the presence of intracellular viral proteins other than the src gene product pp60 src . However, hyperimmunization of mice with hamster or quail fibroblasts transformed by RSV, that express a high level of pp60 src , did not induce transplantation resistance. It is concluded that the expression of both the serologically‐defined VCSA and the transplantation antigen is the result of the interaction of pp60 src with host cell gene product(s) rather than the simple exposure of pp60 src at the outer cell surface.