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Adoptive transfer of immunity induced by semi‐allogeneic hybrid cells, against a murine fibrosarcoma
Author(s) -
Payelle Béatrice,
Poupon MarieFrance,
Lespinats Geneviè
Publication year - 1981
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910270609
Subject(s) - fibrosarcoma , immunity , adoptive cell transfer , immune system , biology , spleen , immunization , immunology , antigen , cellular immunity , peritoneal cavity , antiserum , t cell , genetics , anatomy
Semi‐allogeneic somatic hybrid cells derived from the fusion of a C57BL/6 fibrosarcoma (MCB6–1) and A9 cells (C3H origin) were used to immunize C57BL/6 mice against the parental tumor cells. These hybrid cells expressed H‐2 histocompatibility antigens of both parental cells (H‐2 b and H‐2 k ), and failed to produce tumors in normal C57BL/6 mice. A single i.p. injection of hybrid cells induced anti‐tumor immunity which could be transferred to normal C57BL/6 recipient mice by immune spleen or peritoneal cells; the efficient cells were T cells, as this activity was completely abrogated by treatment with anti‐Thy‐1–2 antiserum and complement. Among immune splenic T cells, only the light‐density T cells, obtained after fractionation on Percoll gradient, were effective in the transfer of immunity. Immunity induced by the hybrid cells was specific for MCB6‐1 parental tumor cells. This immunity could be transferred during two brief periods, 7 to 12 days, and 40 to 50 days, after hybrid cell injection; there appeared to be an intermediate period, 12 to 40 days after immunization, during which no immunity could be transferred. These results suggest a suppressive mechanism implicated during hybrid cell immunization and interacting with the anti‐tumor immune response.