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Fibronectin from human germ‐cell tumors resembles amniotic fluid fibronectin
Author(s) -
Ruoslahti Erkki,
Jalanko Hannu,
Comings David E.,
Neville A. Munro,
Raghavan Derek
Publication year - 1981
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910270606
Subject(s) - fibronectin , fibronectins , immunoperoxidase , amniotic fluid , antiserum , microbiology and biotechnology , pathology , trypsinization , biology , chemistry , cell , biochemistry , immunology , medicine , trypsin , antibody , monoclonal antibody , pregnancy , fetus , genetics , enzyme
Three human germ‐cell tumors established as xeno‐grafts in immunosuppressed mice were shown to produce fibronectin. Immunoperoxidase tissue staining with a specific antiserum revealed human fibronectin in some tumor cells. The cyst fluid which accumulated in the tumors contained human fibronectin as shown by a species‐specific radioimmunoassay. Human fibronectin was isolated from two of the tumor fluids and compared to previously characterized fibronectins. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis and gel electrofocusing showed that the germ‐cell fibronectins resembled amniotic fluid fibronectin with regard to subunit molecular weight and isoelectric point. The distinct features of the amniotic fluid and germ‐cell tumor fibronectin suggest that such fibronectins may provide new oncodevelopmental markers.