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Block of a glioma cell line in S by interferon
Author(s) -
Lundblad D.,
Lundgren E.
Publication year - 1981
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910270604
Subject(s) - thymidine , cell cycle , cell division , cell culture , interferon , dna synthesis , glioma , cell , biology , cell growth , fibroblast , period (music) , microbiology and biotechnology , dna , immunology , cancer research , genetics , physics , acoustics
The human glioma cell line U‐251 MG, with a well‐characterized defect in growth control, was sensitive to the antiproliferative effects of human (fibroblast) interferon (IFN). IFN inhibited exponentially growing cells by increasing the number of cells in the S stage of the cell cycle. At the same time the number of cells in G 0 /G 1 diminished. The rate of thymidine incorporation was decreased during the first cell cycle, with no prolongation of S. However, in synchronized cultures, the wave of cells with a S‐phase content did not decrease over a time period several hours longer than the length of S measured by pulse labelling. Thus we conclude that, at a sufficient dose, the cells were unable to accomplish cell division as they prematurely stopped synthesizing DNA.