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Androgen, estrogen and progestin binding sites in human leukemic cells
Author(s) -
Danel Laurence,
Martin Pierre,
Escrich Edouard,
Tubiaicole,
Fiè Denis,
Saez Simone
Publication year - 1981
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910270602
Subject(s) - myeloid leukemia , leukemia , receptor , medicine , cytosol , endocrinology , estrogen , progestin , dexamethasone , biology , chemistry , biochemistry , enzyme
Sex steroid binding capacity was investigated in malignant cells from 32 patients with acute non‐lymphoblastic leukemia (25 patients with acute myeloid leukemia, 4 with subacute leukemia, 3 with chronic myeloid leukemia in blast crisis) and 30 patients with acute lymphoblastic leukemia. Specific binding of labelled steroids was characterized either by competition assay in cytosol fraction or by whole‐cell incorporation. In some cases further characterization of the receptor complex was attempted by sucrose gradient centrifugation and gel filtration column. The results show the presence of specific binding sites for dexamethasone (22/32 in non ALL and 30/30 in ALL), for estrogens (11/15 in non‐ALL and 5/12 in ALL), for progestins (8/25 in non‐ALL and 5/13 in ALL) and for androgens when RI881 was used as ligand (8/21 in non‐ALL and 5/10 in ALL patients) but only in 1/13 non‐ALL patients and no ALL patients when labelled 5α DHT was used. These results indicate that the blast cells from patients with acute leukemia contain specific proteins binding steroids with a high affinity. Our results for dexamethasone receptors are similar to those described in the literature in ALL and non‐ALL.