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Difference in viral binding between two epstein‐barr virus substrains to a spectrum of receptor‐positive target cells
Author(s) -
Wells Alan,
Koide Norio,
Klein George
Publication year - 1981
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910270308
Subject(s) - virus , epstein–barr virus , virology , receptor , cell culture , biology , lymphoma , microbiology and biotechnology , immunology , genetics
Radio‐labelled Epstein‐Barr virus (EBV) was utilized in a direct binding assay to detect the presence of EBV receptors. The sensitivity of this method was affirmed by the detection of EBV‐receptors on three EBV‐carrying cell lines that have previously been reported as receptor negative. Two laboratory substrains of EBV, derived from the cell lines B95‐8 and P3HR‐1 (designated B and P virus respectively), were tested in the binding assay. The main receptor prototype adsorbed both viral strains without apparent distinction. In contrast, two lines, a Swedish EBV‐negative B‐cell lymphoma (U698) and a virus non‐producer subline of the receptor‐negative P3HR‐1 line, adsorbed P virus selectively but failed to adsorb B virus.