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Lymphocyte‐dependent antibodies to antigen 3.1, a cell‐surface antigen expressed by a subgroup of human melanomas
Author(s) -
Hellström Ingegerd,
Hellström Karl Erik,
Yeh MingYang
Publication year - 1981
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910270305
Subject(s) - antibody dependent cell mediated cytotoxicity , antigen , monoclonal antibody , antibody , microbiology and biotechnology , pan t antigens , biology , immunology , lymphocyte , cytotoxicity , blocking antibody , monoclonal , t lymphocyte , macrophage 1 antigen , virology , in vitro , genetics
Previous work from our laboratory has led to the isolation of a monoclonal IgG 2a mouse antibody, 3.2, which is specific for an antigen, 3.1, that is strongest expressed by a group of human melanomas. We have now demonstrated that this antibody can mediate antibody‐dependent cellular cytotoxicity (ADCC) to human melanoma cells expressing antigen 3.1, and that lymphocytes from human peripheral blood can be used as the source of K cells. A monoclonal IgG 1 antibody, 3.1, to the same determinant of antigen 3.1 gave only weak and inconsistent ADCC.