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EBV infection of mitogen‐stimulated human B lymphocytes
Author(s) -
Einhorn Lena,
Klein Eva
Publication year - 1981
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910270209
Subject(s) - dna synthesis , biology , virus , mitogen activated protein kinase , microbiology and biotechnology , virology , immunofluorescence , epstein–barr virus , dna , antibody , in vitro , immunology , biochemistry , genetics
B lymphocytes from human blood were treated with the mitogen Protein A ( Staph. aureus ), which induces DNA synthesis in, as well as differentiation of, the B lymphocytes. The cells were subsequently exposed to Epstein‐Barr virus (EBV). EBV‐infected and mitogen‐stimulated cells were detected simultaneously, by using a combination of immunofluorescence (for EBNA) and autoradiography (for DNA‐synthesis). In the initial phase (1‐2 days) after addition of the mitogen, stimulated cells were as susceptible to infection as resting cells. Thereafter, their susceptibility decreased. This suggests that, although initiation of DNA‐synthesis does not seem to limit sensitivity to the viral infection, differentiation of the B cells might do so. The intensity and pattern of EBNA‐staining in prestimulated cells differed from that of the controls.