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Destruction of erythroleukemia, myelocytic leukemia and burkitt lymphoma cells by photoactivated protoporphyrin
Author(s) -
Malik Zvi,
Djaldetti Meir
Publication year - 1980
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910260415
Subject(s) - protoporphyrin , protoporphyrin ix , porphyrin , microbiology and biotechnology , chemistry , cytotoxicity , cell culture , biology , in vitro , biochemistry , biophysics , photodynamic therapy , genetics , organic chemistry
The effect of protoporphyrin on erythroid, myeloid and lymphoid leukemic cells and their destruction induced by the photoactivated porphyrin was studied. Friend erythroleukemic cells (FL) and myelocytic leukemic cells (ML) accumulated protoporphyrin in a cap or patch‐like pattern observed by fluorescence microscopy. Photoactivated protoporphyrin induced the appearance of “holes” on the cell membrane demonstrated by scanning electron microscopy. On the other hand, Burkitt lymphoma (BL) and mastocytoma (MS) cells accumulated porphyrin intracellularly around the nuclear envelope and as circular profiles, respectively. Photoactivated protoporphyrin induced development of multiple blebs on the cell membrane, and even complete cell destruction. Cytotoxicity of protoporphyrin at short‐term incubation periods was determined by [ 3 H]thymidine and [ 3 H]uridine incorporation. Protoporphyrin, unexposed to light, reduced the incorporation of both precursors only to a moderate extent. On the other hand, porphyrin‐treated cells exposed to light showed complete inhibition of RNA and DNA synthesis. Long‐term exposure of ML and BL cells to porphyrin in the dark induced a nearly 50 % inhibition of RNA and DNA synthesis. Although the cytotoxic effect of protoporphyrin in the dark was lower than that of photoactivated porphyrin, this may possess a potential activity in vivo even without illumination.