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Changes in the expression of two Epstein‐Barr virus‐associated antigens, EBNA and RANA, during the cell cycle of transformed human B lymphoblasts
Author(s) -
Slovin Susan F.,
Vaughan John H.,
Carson Dennis A.
Publication year - 1980
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910260103
Subject(s) - immunofluorescence , antigen , biology , virus , epstein–barr virus , raji cell , lymphoblast , virology , antibody , microbiology and biotechnology , immunology , cell culture , genetics
Two methods of cell synchronization, density‐dependent arrest and double thymidine block, were used to assign two Epstein‐Barr virus‐associated antigens to different parts of the growth cycle of the human B lymphoblastoid cell lines, WI‐L2 and Raji. The Epstein‐Barr nuclear antigen (EBNA), as detected by anti‐complement immunofluorescence, was maximally expressed during early S phase, decreased during the G 2 and M phases, and was absent in early G 1 . In contrast, the rheumatoid arthritis nuclear antigen (RANA), as detected by anti‐immunoglobulin immunofluorescence with a prototype serum from a patient with rheumatoid arthritis, was maximally expressed during early G 1 phase, progressively decreased during S and early G 2 , and reappeared during late G 2 /M.