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Changes in the expression of two Epstein‐Barr virus‐associated antigens, EBNA and RANA, during the cell cycle of transformed human B lymphoblasts
Author(s) -
Slovin Susan F.,
Vaughan John H.,
Carson Dennis A.
Publication year - 1980
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910260103
Subject(s) - immunofluorescence , antigen , biology , virus , epstein–barr virus , raji cell , lymphoblast , virology , antibody , microbiology and biotechnology , immunology , cell culture , genetics
Abstract Two methods of cell synchronization, density‐dependent arrest and double thymidine block, were used to assign two Epstein‐Barr virus‐associated antigens to different parts of the growth cycle of the human B lymphoblastoid cell lines, WI‐L2 and Raji. The Epstein‐Barr nuclear antigen (EBNA), as detected by anti‐complement immunofluorescence, was maximally expressed during early S phase, decreased during the G 2 and M phases, and was absent in early G 1 . In contrast, the rheumatoid arthritis nuclear antigen (RANA), as detected by anti‐immunoglobulin immunofluorescence with a prototype serum from a patient with rheumatoid arthritis, was maximally expressed during early G 1 phase, progressively decreased during S and early G 2 , and reappeared during late G 2 /M.