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Enrichment of suppressor cell activity by hydrocortisone: Suppression of in vitro activation of splenocytes from mice bearing lewis lung carcinoma
Author(s) -
Clerici Enrico,
Schechter Bilha,
Feldman Michael
Publication year - 1980
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910250308
Subject(s) - splenocyte , lewis lung carcinoma , spleen , concanavalin a , in vitro , in vivo , biology , population , suppressor , cancer research , endocrinology , microbiology and biotechnology , medicine , immunology , cancer , biochemistry , metastasis , environmental health
Suppressor cells in spleens of mice bearing the Lewis lung carcinoma (3LL) suppressed the anti‐tumor immune response in vivo but not in vitro . The hydrocortisone (HC)‐resistant fraction of spleen cells from tumor‐bearing mice (TBM) was, on the other hand, suppressive in vitro , due to enrichment for HC‐resistant suppressor cells. This cellular fraction suppressed the tumor‐induced differentiation of memory cells as well as the tumor‐independent in vitro activation of TBM spleen cells. The in vitro lymphoproliferative response of TBM spleen cells to mitogens was also subjected to suppression: these cells showed a lower response to a mitogen such as Concanavalin A (Con A) and were capable of suppressing the response of normal spleen cells to this mitogen. HC treatment of TBM 2 days prior to spleen harvest enriched the splenic population for suppressor cells that suppressed Con A‐induced activation.