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Retinoids inhibit the mitogenic activity of tumourpromoting phorbol esters on human lymphocytes
Author(s) -
Abb Jochen,
Deinhardt Friedrich
Publication year - 1980
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910250215
Subject(s) - phorbol , retinoic acid , lymphocyte , tretinoin , mechanism of action , chemistry , retinyl acetate , mode of action , inhibitory postsynaptic potential , biology , biochemistry , protein kinase c , endocrinology , in vitro , immunology , enzyme , gene
The tumour‐promoting agents 12‐0‐tetradecanoyl‐phorbol‐13‐acetate (TPA) and phorbol‐12,13‐dibutyrate (PDB) 1 are potent mitogens for human peripheral blood lymphocytes. In contrast, the non‐cocarcinogenic substance phorbol lacks lymphocyte‐activating properties. Non‐toxic levels of retinoic acid (RA) or retinyl acetate (RAt) inhibit the phorbol‐ester‐induced lymphocyte proliferation. Selective inhibition of phorbol‐ester‐stimulated lymphocyte blastogenesis required the near‐concurrent addition of retinoids. Differences in the sensitivity of phorbol‐ester‐stimulated lymphocyte subpopulations to the antagonistic action of RA or RAt, respectively, suggest that the inhibitory effect of retinoids may not be due to a common mode of action. Lymphocyte cultures may provide a useful model system for studies of the mechanisms of action of both phorbol esters and retinoids.