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H‐2 associated resistance to leukaemia transplantation: Natural killing in vivo
Author(s) -
Carlson George A.,
Melnychuk David,
Meeker Mary Jane
Publication year - 1980
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910250115
Subject(s) - in vivo , spleen , bone marrow , transplantation , biology , immunology , thymidine , in vitro , microbiology and biotechnology , medicine , genetics
Natural resistance in vivo was studied by injecting non‐immunized mice with leukaemia cells prelabelled with the thymidine analogue 131 I‐iododeoxyuridine. There was a decrease in the survival of the leukaemia cell inoculum as determined by whole‐body gamma counting, and a failure of the leukaemia cells to survive in the spleens of mice which were not H‐2 identical with the transplant. H‐2‐associated resistance could be measured within 24 h of leukaemia inoculation and was strongest in the spleen and absent from the liver. Although all strains of mice tested were able to resist H‐2 non‐identical cells, resistance in irradiated (800‐900 R) mice was restricted to certain strains and their F 1 hybrids, notably those of the C57BL family. Resistance in both non‐irradiated and irradiated mice was not due to classical immunological rejection. Mice with either genetic or induced T‐cell deficiency showed full resistance, and circulating preformed antibody could not account for the rejection observed. Treatment with silica or with 89 Sr abrogated natural resistance in non‐irradiated as well as irradiated animals; these treatments had previously been shown to abolish both bone‐marrow graft rejection in irradiated mice and in vitro natural killing. Resistance against leukaemia transplantation in irradiated C57BL mice appeared to depend on Hh‐I (H‐2D) incompatibility between the host and the graft, again suggesting that bone‐marrow graft rejection, and perhaps natural killer activity, is a subset of a more general paraimmune or non‐adaptive rejection mechanism.