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Rauscher leukemia virus‐induced tumor antigens: Complete separation from gp70, p30 and H‐2
Author(s) -
Alaba Olusola,
Rogers M. J.,
Law L. W.
Publication year - 1979
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910240514
Subject(s) - ctl* , antigen , leukemia , microbiology and biotechnology , cytotoxic t cell , in vitro , virus , biology , histocompatibility , murine leukemia virus , chemistry , pan t antigens , virology , immunology , human leukocyte antigen , biochemistry
The possibility that some or all of the viral proteins, gp 70, p 30, and the histocompatibility antigen, H‐2, function as the tumor‐specific transplantation antigen (TSTA) of the R‐MuLV‐induced leukemia, RBL‐5, and also in the secondary in vitro induction of cytotoxic T lymphocytes (CTL), was investigated. The antigen was obtained by isolating the plasma membranes of RBL‐5 cells and solubilizing with sodium deoxycholate (DOC) followed by gel filtration chromatography. A fraction containing excellent tumor‐rejection activity but low amounts of gp 70, p 30 and H‐2 was chromatographed on goat anti‐gp 70 goat anti‐p 30 and sheep anti‐H‐2 b immunoaffinity columns. The data obtained indicate that gp 70, p 30 or H‐2 do not function as TSTA of RBL‐5 leukemia, individually or as a complex. Similarly, the antigen responsible for the specific secondary induction of CTL in vitro is distinct from these three proteins.