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Establishment and characterization of human B‐lymphocytic lymphoma cell lines (balm‐3, ‐4 and ‐5): Intraclonal variation in the B‐cell differentiation stage
Author(s) -
Lok MingSwang,
Koshiba Hirofumi,
Han Tin,
Abe Syuiti,
Minowada Jun,
Sandberg Avery A.
Publication year - 1979
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910240509
Subject(s) - lymphoma , biology , cell culture , b cell , cell , population , epstein–barr virus , surface immunoglobulin , b cell lymphoma , primary effusion lymphoma , antigen , antibody , virus , immunology , genetics , medicine , environmental health
This study describes the establishment of three non‐Burkitt B‐lymphoma cell lines (BALM‐3, BALM‐4 and BALM‐5) originating from the pleural effusion of a patient with a poorly differentiated diffuse lymphocytic lymphoma. The cells of BALM‐3, ‐4 and ‐5 exhibited a number of properties which distinguish them from the usual B‐cell type lymphoblastoid cell lines. Thus, they lacked the Epstein‐Barr virus genome and had abnormal chromosome constitutions including a 14q+ marker. The presence of the identical surface immunoglobulin isotypes (γ and χ chain determinants), and la‐like B‐cell‐associated antigen in the cultured cells and in the “fresh” lymphoma cells in vivo was demonstrated. These findings strongly suggested that these cell lines have B‐cell characteristics and were derived from the original tumor cell population. BALM‐5 cells, however, showed somewhat different growth, cell surface marker profile and functional characteristics compared to those of BALM‐3, and ‐4 cells. These variations suggest that the BALM‐5 cells were probably at different stages of B‐cell maturation than those of BALM‐3 and ‐4, even though all three cell lines (established in three separate flasks) originated from the cells of the same pleural effusion of a lymphoma with monoclonal B‐cell characteristics.